DNA-loaded lipid nanoparticles are poised to bring gene therapy to common chronic diseases

Researchers at the University of Pennsylvania have developed a new process that transports DNA into cells using lipid nanoparticles. Unlike messenger RNA (mRNA), DNA remains active in cells for months, or even years, and can be programmed to work only in targeted cells. But past attempts to use lipid nanoparticles to deliver DNA failed, because DNA can trigger severe immune reactions. The researchers discovered that by adding a natural anti-inflammatory molecule, called nitro-oleic acid, to the lipid nanoparticles, these immune reactions could be eliminated. With this advancement, treated cells produced intended therapeutic proteins for about six months from a single dose – much longer than the few hours seen with mRNA therapies.